The exisiting Manor dates back to the early 17th century, however the East Wing was built in the 14th century and it is believed there has been a building here since at least AD 872. Once owned by Edward the Confessor, the building is noted in the Domesday Book and is also mentioned in the will of King Alfred the Great in 885. The Manor was farmed by abbots of Quarr Abbey and for over 400 years was in their possession. In the reign of Henry VIII the manor came into the hands of the Crown following the dissolution of the monasteries and was leased to the Leigh family for 70 years. In 1560, John Leigh, who was 13 at the time, is believed to have smothered his father, Barnaby, with a pillow. John's young sister, Annabelle, witnessed this, so he threw her from a window to her death. Charles I gave the manor to trustees for the payment of his debts and it was sold to two London Merchants. Charles visited the manor on several occasions and stayed there before being imprisoned in Carisbrooke Castle. Both Queen Victoria and Queen Mary often visited the manor, while more recently it has been open to the public as a museum. Arreton Manor is said to have many restless spirits, including the ghost of fair-haired Annabelle, a mysterious lady in a purple dress, and many sightings of a silvery-grey figure. A woman in a red dress has also been spotted while monks have been seen walking through walls in the Dining Room, as well as nodding in silent prayer in the 12th-century part of the house.

The investigation began with the usual walk around with Derek, as he connected with a large woman he felt had a link with The Hague as well as the names Edward Bryan and Ami Tetchnook. None of these could be verified, although Margaretta van Hesse of the Hague had married Thomas Culpeper in 1659 who owned the manor but who resided at Carisbrooke Castle. David Wells connected with the name Henry Sommerhill (not verified) while both mediums suggested that the spirit of a little girl resided there, possibly related to the legend of the child who after witnessing her father being murdered was thrown from an upstairs window to her death. The vigils suggested some fairly significant poltergeist activity, with the team initially witnessing a paperback book (coincidentally titled 'Ghosts of the Isle of Wight') appearing to fly from the sideboard onto the floor with no apparent explanation, although the movement was not captured on camera. Later in the same room it also appears that a large plate falls from the table onto the floor, again with no explanation but also without camera evidence of it moving by itself. In a final vigil in the attic Karl and Stuart are startled by a loud noise, as it appears that the side of a sofa falls onto the floor. Upon inspection it is identified that this section of the sofa is held together by a toggle and a rope, however the toggle appears to have vanished. Exploring the stairwell near the sofa the pair then appear to capture a loud knock on camera, as if something has fallen onto the wooden stairs, and Karl remarkably discovers the large wooden toggle. Again however the movement of the object was not captured on camera, and the whereabouts of Karl cannot be verified at the time of the event as the camera is focused solely upon Stuart. Other activity from the vigils included Cath suggested she had been grabbed around the legs, a number of light anomalies as well as some interesting auditory phenomena.